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Colloidal Gold Conjugation

What it is

Conjugation is the process of attaching detection antibodies to colloidal gold nanoparticles (typically ~40 nm), producing the colored label that makes the test line visible.

How it works

Gold nanoparticles are synthesized and then passively adsorb antibodies via electrostatic and hydrophobic interaction; the conjugate is stabilized, blocked and dispensed onto the conjugate pad. Batch-to-batch control of particle size and antibody density determines signal intensity and consistency.

Why it matters

Conjugate quality directly affects sensitivity and the clarity of the result line — a key QC point and a common source of batch variation that buyers should ask about.

Key components

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Written by the Progene Bio Technical Team · Last reviewed: 2026-09-12