Colloidal Gold Conjugation
What it is
Conjugation is the process of attaching detection antibodies to colloidal gold nanoparticles (typically ~40 nm), producing the colored label that makes the test line visible.
How it works
Gold nanoparticles are synthesized and then passively adsorb antibodies via electrostatic and hydrophobic interaction; the conjugate is stabilized, blocked and dispensed onto the conjugate pad. Batch-to-batch control of particle size and antibody density determines signal intensity and consistency.
Why it matters
Conjugate quality directly affects sensitivity and the clarity of the result line — a key QC point and a common source of batch variation that buyers should ask about.
Key components
- Gold nanoparticle synthesis (uniform ~40 nm)
- Antibody adsorption (passive conjugation)
- Stabilization and blocking
- Conjugate pad dispensing and drying
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Written by the Progene Bio Technical Team · Last reviewed: 2026-09-12