Fluorescence Immunoassay (FIA)

What it is

Fluorescence immunoassay uses a fluorescent label instead of colloidal gold and is read by a dedicated analyzer, producing quantitative (not just qualitative) results.

How it works

A fluorescent conjugate binds the analyte and is captured at the test line; a fluorescence analyzer measures the emitted signal intensity, which is converted to a concentration via a calibration curve.

Why it matters

FIA upgrades rapid tests from qualitative triage to quantitative measurement — relevant for cardiac markers, hormones and inflammation markers where a numeric result adds clinical value.

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